Asadullah Khatoun endowed 400 manuscripts to Astan Quds Razavi in 1067 AH. This collection has been the biggest library endowed before the delivery of a collection of more than 700 manuscripts that Nader Shah Afshar in 1145 AH, that he had brought to Iran from the Indian War. This research was done about this library and its endower. That is, to see what information the list of manuscripts of Asadullah Khatoun entails. Another aim of the study was to see what information we can get from the manuscripts about the endower and the origin of his endowed library.  The name of the endower and his lineage, according to the manuscripts, is in these words “the waqef is Asadullah ibn Sheikh Mohammad Mu’min (known as bn Khatoun al-Mashhadi Ameli). Another thing that this study answers is that whether there is a kin relationship to Sheikh Mohammad Khatoun (1059 AH), the leader of the government of Abdullah Qutb Shah in South India and to see if it is possible that these endowed manuscripts belonged to his personal library. The results showed that the 400 copies that have been endowed to Astan Quds Razavi belong to Alami Fahami Sheikh Mohammad bin Khatoun, and the collection has been endowed the library of Astan Quds Razavi by his son. The person has been a leader and vicegerent of Heider Abad kingship for about 20 years of the 46-year period of Shia government of Sultan Abdullah Qutb Shah. Mohammad Khatoun was a pupil of Sheikh Bahaei and Mir Mohammad Mo’men Estrabadi and has established a famous religious school in the south of India.