Mashhad as the capital of Afsharid dynasty and holding the holy shrine of Imam Reza, experienced many ups and downs over Afsharid period. All trustees of Astan Quds were of Sadat Razavi and Hosseini families, but their power and authority diminished over time, as the dominant power of the Afsharid dynasty was settled in Mashhad and the kings could control and interfere more easily with different matters of Astan Quds Razavi. Nader Shah and his successors usually made decisions for the trustees on the way of managing Astan Quds, on the way of its cooperation with other organizations and government agents, and on other significant issues. The trustees’ activities and power were limited and the number of employees was reduced in this period. Holding Shias’ specific religious and ritual ceremonies like those common in the Safavid era became more confined. The religious ceremonies of Muslims, especially Eid al-Fitr, Eid al-Adha, and Iranians’ national celebration (i.e., Eid Nowruz) were hold magnificently. Nader Shah specifically emphasized on building and developing religious places, maintained his relationship with Shia scholars and employed them in managing the administrative organization of the Holy Shrine and Jame’ Mosques. This study tried to explore Nader’s religious policies and behavior using the mentioned issues and the survived documents from the period.